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991.
Mekibib Kassa Saïd Abdellati Lieselotte Cnops Bruno C. Bremer Hinckel Arega Yeshanew Wasihun Hailemichael Florian Vogt Wim Adriaensen Pascal Mertens Ermias Diro Johan van Griensven Dorien Van den Bossche 《PLoS neglected tropical diseases》2020,14(12)
Diagnosis of a first-time visceral leishmaniasis (VL) infection in Ethiopia is established by use of a rapid diagnostic test (RDT) detecting antibodies against rK39, direct agglutination test (DAT) and microscopy according to the national algorithm. The performance of individual tests and algorithm is variable and depends on several factors, one being HIV status. Limited data are available on the performance of tests in VL-HIV coinfected patients.Assessment of the performance of DAT (ITM-A), rK39 ELISA (Serion) and six RDT (Onsite Leishmania Ab CTK, Antigen ICT Xinjier, IT Leish Biorad, Kalazar Detect Inbios, rK39 IgG1 Coris, rk28 IgG1 Coris) for the diagnosis of VL was done on a panel of 91 stored serum and plasma samples of ‘first-episode’ suspected VL patients, with HIV coinfection (n = 51) and without (n = 40). A combined reference standard was used: either positive microscopy on tissue aspirates, or in case of negative microscopy, positive PCR results on the aspirate slide. Additionally, endemic healthy controls (n = 20), non-endemic controls (n = 10) and patients with confirmed malaria infection (n = 10) were tested for specificity evaluation. Sensitivities ranged from 69.2% for DAT (applied cut-off ≥ 1/3200) to 92.2% for the Onsite RDT, whereas specificities ranged from 20.0% for Kalazar Antigen ICT to 100% for IT Leish and rK39 IgG1. Sensitivities from all assays decreased upon stratification according to HIV status but was only significantly different for rK39 Serion ELISA (p-value 0.0084) and the Onsite RDT (p-value 0.0159).In conclusion, performance of commercially available assays for VL on samples from Northern-Ethiopian patients varied widely with a substantial decrease in sensitivity in the VL-HIV coinfected group. Clear guidelines on minimal performance criteria of individual tests and algorithms are needed, as well as which reference standard should be used to determine the performance. 相似文献
992.
Wim J. van der Steen 《Acta biotheoretica》1998,46(4):369-377
Research in behaviour genetics uncovers causes of behaviour at the population level. For inferences about individuals we also need to know how genes and the environment affect phenotypes. Behaviour genetics fosters a biased view of individual behaviour since it identifies the environment with psychosocial factors and disregards ecology. 相似文献
993.
994.
Per Klemm Wim Gaastra Moyra M. McConnell Henry R. Smith 《FEMS microbiology letters》1985,26(2):207-210
Abstract The CS2 fimbrial antigen was isolated by salt and isoelectric precipitation and by column chromatography. The purified antigen was free of other fimbrial proteins present on the same bacterial strain. Analysis of the N-terminal amino acid sequence indicated extensive homology with the CFA1 fimbrial antigen, which was surprising since the two proteins do not show any immunological cross reactivity. It could therefore be concluded that the N-terminal parts of these fimbrial proteins are not located on the surfaces of the proteins and might be involved in conservation of the structural integrity of these proteins. 相似文献
995.
The nucleotide sequence of the gene encoding the K99 subunit of enterotoxigenic Escherichia coli 总被引:9,自引:0,他引:9
Abstract The nucleotide sequence of the gene encoding the K99 fimbrial subunit of enterotoxigenic Escherichia coli was determined. It appeared that the subunit is composed of 159 amino acid residues preceded by a N-terminal signal sequence of 22 amino acid residues. The secondary structure of the mature K99 polypeptide and the location of potential antigenic determinants were predicted. A comparison was made between the amino acid sequence of the K99 subunit and the subunits of other fimbrial adhesins. 相似文献
996.
Purification and properties of a neutral invertase from the roots of Cichorium intybus 总被引:1,自引:0,他引:1
Multiple activity peaks of neutral invertase (EC 3.2.1.26) were found in chicory roots ( Cichorium intybus L. var. foliosum cv. Flash). The main activity peak was purified by a combination of anion-exchange chromatography, hydrophobic interaction chromatography, chromatofocusing and gel filtration. This protocol produced a 77-fold purification and a specific activity of 1.6 μmol (mg protein)−1 min−1 . The mass of the enzyme was 260 kDa as estimated by gel filtration and 65 kDa on SDS-PAGE. Optimal activity was found between pH 7 and 7.5. The purified enzyme exhibited hyperbolic saturation kinetics with a Km between 10 and 20 mM for sucrose. No other products than glucose and fructose could be detected. Raffinose was hydrolyzed at a rate of 2.4% relative to sucrose whereas the enzyme did not hydrolyze maltose, cellobiose, trehalose, 1-kestose, 1.1-nystose or inulin. Neutral invertase activity was completely inhibited by HgCl2 and AgNO3 and partially inhibited by CoCl2 , and ZnSO4 (1 mM). Pyridoxal phosphate (Ki ≅ 500 μ M ), Tris (Ki ≅ 1.2 m M ), glucose and fructose (Ki ≅ 16 m M ) were strong inhibitors of the enzyme. Fructose and Tris behaved as competitive inhibitors. A possible role for the enzyme's activity in vivo is discussed. 相似文献
997.
A. Coomans K. F. Vaas P. J. Roos W. L. van Utrecht P. J. Schroevers Wim Vader 《Hydrobiologia》1977,54(3):279-280
Ohne Zusammenfassung 相似文献
998.
Louise A.M. de Haan Geraldine A. Willshaw Bernard A.M. van der Zeijst Wim Gaastra 《FEMS microbiology letters》1991,83(3):341-346
A DNA fragment that can functionally substitute for cfaD, the positive regulatory gene involved in expression of CFA/I fimbriae, has recently been cloned from an Escherichia coli strain of serotype O167:H5 that produces CS5 fimbriae. Nucleotide sequence determination showed that the fragment contained a gene, csvR (Coli Surface Virulence factor Regulator) homologous to the cfaD gene, which encoded for a protein of 301 amino acid residues. The csvR gene was found to be located between two different insertion sequences. Comparison of the amino acid sequence of the CsvR and CfaD proteins showed that CsvR is 34 amino acid residues longer at the C-terminus and, in the sequence, it also contains an insertion of two amino acid residues. The similarity between CfaD and Rns, the positive regulator of CS1 and CS2 expression, is much higher (97%) than between CsvR and CfaD (87%). This is reflected by the fact that the level of expression of CFA/I fimbriae induced by CsvR is not as high as when expression is induced by CfaD or Rns. 相似文献
999.
1000.
Grime (1979) in a recently developed theory distinguished three basic plant strategies: stress tolerance,ruderality and competition. He relates them to environments characterized in terms of stress and disturbance. Classifications of strategies and environments both are ultimately defined in terms of production. This tends to make the theory tautological. If the theory is to make sense, environments had better be defined in independent terms. 相似文献